RECORD NO.:  95220697
       AUTHOR:  Gunaratne PH; Nakao M; Ledbetter DH; Sutcliffe JS; Chinault 
                AC
      ADDRESS:  Department of Molecular and Human Genetics, Baylor College 
                of Medicine, Houston, Texas 77030, USA.
        TITLE:  Tissue-specific and allele-specific replication timing 
                control in the imprinted human Prader-Willi syndrome region.
       SOURCE:  Genes Dev (FN3), 1995 Apr 1; 9 (7): 808-20
     LANGUAGE:  English
 COUNTRY PUB.:  UNITED STATES
 ANNOUNCEMENT:  9507
    PUB. TYPE:  JOURNAL ARTICLE
     ABSTRACT:  To examine the relationship between replication timing and 
                differential gene transcription in tissue-specific and 
                imprinted settings we have studied the replication timing 
                properties of the human Prader-Willi syndrome (PWS) region 
                on human chromosome 15q11-13. Interphase fluorescence in 
                situ hybridization with an overlapping series of cosmid 
                clones was used to map a PWS replication timing domain to a 
                500- to 650-kb region that includes the SNRPN gene. This PWS 
                domain replicates late in lymphocytes but predominantly 
                early in neuroblasts, with replication asynchrony observed 
                in both tissues, and appears to colocalize with a 
                genetically imprinted transcription domain showing prominent 
                expression in the brain. A 5- to 30-kb deletion in the 5' 
                region of SNRPN results in the loss of late replication 
                control of this domain in lymphocytes when the deleted 
                chromosome is inherited paternally. This potential allele-
                specific replication timing control region also appears to 
                colocalize with a putative imprinting control region that 
                has been shown previously to abolish the expression of three 
                imprinted transcripts in this same region.
MESH HEADINGS:  *DNA Replication; *Genomic Imprinting; Prader-Willi 
                Syndrome--genetics (*GE); Ribonucleoproteins, Small Nuclear--
                genetics (*GE); Cells, Cultured; Chromosomes, Human, Pair 
                15; Lymphocytes--metabolism (ME); Neurons--metabolism (ME); 
                Sequence Deletion; Transcription, Genetic; Tumor Cells, 
                Cultured; Female; Human; Male; Support, Non-U.S. Gov't; 
                Support, U.S. Gov't, P.H.S.
  GENE SYMBOL:  SNRNP
CHEMICAL SUBS:  0 (Ribonucleoproteins, Small Nuclear)
    GRANT NO.:  8D20619
 STANDARD NO.:  0890-9369
        DATES:  Entered 950509