R01HD32179       HAY, ROBERT J             HUMAN FETAL TISSUE AND CELL CULTURES
-PROJECT NUMBER......1 R01 HD32179-01
                                     FY  94    HAY, ROBERT J
IRG/INTRAMURAL UNIT..SRC                       AMERICAN TYPE CULTURE COLLECTI
AWARD AMOUNT.........   $233,141               12301 PARKLAWN DRIVE
                                               ROCKVILLE, MD  20852
PERFORMING ORGANIZATION: AMERICAN TYPE CULTURE COLLECTION
TITLE   HUMAN FETAL TISSUE AND CELL CULTURES FOR RESEARCH

ABSTRACT:

A feasibility study is proposed to explore the utility of various tissues
from human abortuses for transplantation and related research.
Specifically, aborted fetuses plus parental blood samples and all relevant
clinical data will be obtained through a local hospital complex.  Whenever
possible, pancreas, skin and muscle, liver, kidney, cartilage and lung
tissues will be removed, dissociated and subfractionated for
cryopreservation and cultivation trials in vitro.  Existing protocols for
these manipulations will be compared and improved upon as required.
Clonal culture, cell aggregate maintenance techniques and use of feeder
cell populations will be utilized where appropriate to develop
quantitative comparative data. Histological and biochemical assays will be
applied both to evaluate separation/cultivation methods and to identify
optimal culture conditions for maintaining functional cells.  Cell
populations will be characterized with regard to expression of surface HLA
and other significant transplantation antigens, and profiles will be
compared to those of the parental donors.  Tissue and cell culture
populations will be examined for infections with bacteria, fungi,
mycoplasma, HIV, CMV, hepatitis B and other viruses. Cytogenetic analyses
will confirm the normal diploid status. Pertinent cell stocks will be made
available to foster collaboration with other institutions.

CRISP INDEXING TERMS FROM CRISP THESAURUS:
14654300P   human embryo /fetus tissue
24044640P   induced abortion
29355444P   tissue /cell culture
29365148P   tissue resource /registry
12561766S   cytogenetics
28713303S   cryopreservation
29355928S   histocompatibility typing
29356327S   histocompatibility antigen
40000353S   transplantation immunology
06307520T   polymerase chain reaction
14652234T   human subject
15549780T   western blotting
23230610T   affinity chromatography
40000320T   SDS polyacrylamide gel electrophoresis